Single Cell Transcriptomics: Methods and Protocols

Single Cell Transcriptomics: Methods and Protocols

Author
Raffaele A. Calogero, Vladimir Benes
Publisher
Humana Press
Language
English
Year
2022
Page
393
ISBN
1071627554,9781071627556
File Type
pdf
File Size
10.5 MiB

This volume provides up-to-date methods on single cell wet and bioinformatics protocols based on the researcher experiment requirements. Chapters detail basic analytical procedures, single-cell data QC, dimensionality reduction, clustering, cluster-specific features selection, RNA velocity, multi-modal data integration, and single cell RNA editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Single Cell Transcriptomics: Methods and Protocols aims to be a valuable resource for all researchers interested in learning more about this important and developing field.

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