Fluorescent analogs of biomolecular building blocks : design and applications

Cover; Title Page; Copyright; Contents; List Of Contributors; Preface; Chapter 1 Fluorescence Spectroscopy; 1.1 Fundamentals Of Fluorescence Spectroscopy; 1.2 Common Fluorescence Spectroscopy Techniques; 1.2.1 Steady-state Fluorescence Spectroscopy; 1.2.2 Time-resolved Fluorescence Spectroscopy; 1.2.3 Fluorescence Anisotropy; 1.2.4 Resonance Energy Transfer And Quenching; 1.2.5 Fluorescence Microscopy And Single Molecule Spectroscopy; 1.2.6 Fluorescence-based In Vivo Imaging; 1.3 Summary And Perspective; References. Chapter 2 Naturally Occurring And Synthetic Fluorescent Biomolecular Building Blocks; 2.1 Introduction; 2.2 Naturally Occurring Emissive Biomolecular Building Blocks; 2.3 Synthetic Fluorescent Analogs Of Biomolecular Building Blocks; 2.3.1 Synthetic Emissive Analogs Of Membranes Constituents; 2.3.2 Synthetic Emissive Analogs Of Amino Acids; 2.3.3 Synthetic Emissive Analogs Of Nucleosides; 2.4 Summary And Perspective; References; Chapter 3 Polarized Spectroscopy With Fluorescent Biomolecular Building Blocks; 3.1 Transition Moments; 3.2 Linear Dichroism; 3.3 Magnetic Circular Dichroism. 3.4 Forster Resonance Energy Transfer (fret); 3.5 Fluorescence Anisotropy; 3.6 Fluorescent Nucleobases; 3.7 Fluorescent Peptide Chromophores; 3.8 Site-specific Linear Dichroism (ssld); 3.9 Single-molecule Fluorescence Resonance Energy Transfer (smfret); 3.10 Single-molecule Fluorescence-detected Linear Dichroism (smfld); References; Chapter 4 Fluorescent Proteins: The Show Must Go On!; 4.1 Introduction; 4.2 Historical Survey; 4.3 Photophysical Properties; 4.3.1 Absorption Properties And Color Hue Modification; 4.3.2 Chromophore Formation; 4.3.3 Fluorescence Color And Dynamics. 4.3.4 Directional Properties Along With Optical Transitions; 4.3.5 Energy Transfer And Energy Migration; 4.4 Photochemical Reactions; 4.4.1 Excited-state Proton Transfer (espt); 4.4.2 Isomerization Reactions: Reversible Photoswitching; 4.4.3 Photoconversion: Irreversible Bond Rupture; 4.4.4 Other Photochemical Reactions; 4.5 Ion Sensitivity; 4.5.1 Ground-state Equilibria Of Protonation States; 4.5.2 Quenching By Small Ions; 4.6 Relation Microscopy-spectroscopy For Fluorescent Proteins; 4.6.1 Brightness Alteration From Cuvette To Microscopic Experiments; 4.6.2 Lessons From Microspectrometry. 4.6.3 Tools For Advanced Microscopic Techniques; 4.7 Prospects And Outlook; Acknowledgments; References; Chapter 5 Design And Application Of Autofluorescent Proteins By Biological Incorporation Of Intrinsically Fluorescent Noncanonical Amino Acids; 5.1 Introduction; 5.2 Design And Synthesis Of Fluorescent Building Blocks In Proteins; 5.2.1 Extrinsic Fluorescent Labels; 5.2.2 Intrinsic Fluorescent Labels; 5.2.3 Extrinsic Labels Chemically Ligated Using Cycloaddition Chemistry; 5.2.4 Modification Of The Genetic Code To Incorporate Ncaas; 5.3 Application Of Fluorescent Building Blocks In Proteins. 5.3.1 Azatryptophans. Edited By Marcus Wilhelmsson, Yitzhak Tor. Includes Bibliographical References And Index. Mode Of Access: World Wide Web.