PCR protocols in molecular toxicology

PCR protocols in molecular toxicology

Author
Vanden Heuvel, John P
Publisher
CRC Press
Language
English
Year
1998
Page
237
ISBN
9780849333446,084933344X
File Type
pdf
File Size
40.3 MiB

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression
Differential display cloning
Cloning and library screening by PCR
Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
Basic, non-PCR based molecular biology methods

PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

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