Epigenetic Reprogramming During Mouse Embryogenesis: Methods and Protocols

Epigenetic Reprogramming During Mouse Embryogenesis: Methods and Protocols

Author
Katia Ancelin, Maud Borensztein
Publisher
Springer US;Humana
Language
English
Edition
1st ed.
Year
2021
Page
XI, 318
ISBN
9781071609576,9781071609583
File Type
pdf
File Size
10.8 MiB

This volume explores the latest techniques used to study and understand chromatin reprogramming in embryos and germ cells. Various culture systems are presented, which consist of invaluable tools to investigate many developmental processes. This book also looks at methods for direct examination of DNA, RNA, and proteins in embryos, along with low-input and single-cell assays for exploring these features at the genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Epigenetics Reprogramming During Mouse Embryogenesis: Methods and Protocols is a valuable resource for any scientist and researcher looking to make new discoveries in this fascinating field of chromatin reprogramming.

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